Enzyme lab

Enzyme Lab Worksheet

Hypothesis: I predict that the higher the PH is the faster the enzyme reaction will be.

Independent Variable: PH levels

Dependent Variable: Height of the foam

Controlled Variables: The amount of enzyme extract, water and hydrogen peroxide

Justification of hypothesis: I choose my hypothesis, because i believed there would be more bubbles from the base formulas rather than acid ones. I also believed that the enzymes would receive more reactions from base rather than acid

Materials (Your Team’s Experiment):

• Beaker
• mortar
• pestle
• bindweed
• test tube
• ruler
• timer
• hydrogen peroxide
• pipet
• paper towel
• scissors

Procedure: First you gather bindweed. Once you have gathered a good amount you cut of the leaves. Then you use the mortar and pestle to mash up the bindweed. Add 20 ml of water to the bind. After the bindweed has turned into a liquidy substance put a paper towel over a beaker and slowly pour the bindweed substance through to paper towel. Squeeze the paper towel to extract any left over liquid. Then pour the substance into a test tube. Add 0ne and a have ml of peroxide to the extract. The extract acts as a substrate. Then Observe for 30 seconds. Repeat with a solution of extract, peroxide and hydrochloric pH 3-4 sodium hydroxide pH 10 and hydroxide pH 12.

Data and Results:  

Conclusions: As a result to our lab my hypothesis was proven correct. The higher the pH levels are the faster the enzyme react. During our first lab attempt we failed to properly insert the different PH levels and diluted bindweed extract. This caused the enzyme reaction to be slower. In our second attempt we made sure to separate the different pH levels. By doing this is caused a better reaction. Resulting in larger foaming levels and in a shorter time. This means that the higher the PH levels the faster the enzymes react.